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Description
YPD PlusThe outgrowth step in yeast transformation protocols is often critical for increasing overall yeast transformation efficiencies. Following treatment of yeast with lithium acetate and polyethylene glycol solutions, it is often necessary to add medium to allow for a period of outgrowth, which normally results in increased cell survival. This is useful when attempting to maximize transformation efficiencies for library screening or transforming yeast
The outgrowth step in yeast transformation protocols is often critical for increasing overall yeast transformation efficiencies. Following treatment of yeast with lithium acetate and polyethylene glycol solutions, it is often necessary to add medium to allow for a period of outgrowth, which normally results in increased cell survival. This is useful when attempting to maximize transformation efficiencies for library screening or transforming yeast with multiple plasmids. YPD Plus is a special formulation of conventional YPD outgrowth medium that increases yeast transformation efficiencies by > 50%. YPD Plus is recommended for mutant yeast strains exhibiting poor growth characteristics that are not amenable to transformation. Simply supplement a yeast transformation reaction mixture with YPD Plus to achieve consistent increases in yeast transformation efficiencies.Shipping Notes
- Free Standard Shipping on $100+ Orders to the USA.
- Except Preorder products are shipped in 48 hours.
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4.7 ★★★★★
Based on 309 reviews
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Product Reviews
★★★★★ 5
Slip-on ready and great quality
Size: 9, Color: Black
These shoes were stuff for the first half hour but then feel great. Well worth the chance and money. Great quality and slip-on easy
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Reviewed in the United States on May 22, 2026
★★★★★ 5
Great buy
Size: 11, Color: Tan
Great fit comfortable look good
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Reviewed in the United States on May 12, 2026
★★★★★ 5
Great shoes
Size: 14 Wide, Color: Black
My shoes look good and feel good and now I’m going to purchase the brown pair I saw
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Reviewed in the United States on April 24, 2026
★★★★★ 4
Don’t buy for restaurant industry
Size: 10.5, Color: Black, Size: 10.5, Color: Black
Good looking shoes, fairly comfortable and good value based on the description shared, so I don’t feel it’s fair to rate them lower than 4 stars. I had high hopes for them based on previous reviews from others saying they wore them in the food and beverage industry that mentioned their quality and durability, however I strongly recommend looking elsewhere if you do. I purchased these to wear working in a full service upscale restaurant and in less than two months have managed to have torn the material on the middle exterior section and the bottoms of the shoes are already completely worn down. Good design, but the durability needs to be improved if they’re to hold up for that industry.
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Reviewed in the United States on July 18, 2025
★★★★★ 1
DO NOT BUY (lasted less than 7 months)
Size: 11, Color: Black, Size: 11, Color: Black
I bought these for work as a bartender in August of 2025. By December, the tops were scuffed, frayed, and peeling beyond repair, and by February, the soles were coming off. I’ve never had a pair of shoes with such low quality.
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Reviewed in the United States on April 11, 2026