SKU: 13407734266

fT3 (Free Triiodothyronine) ELISA Kit

Sale price$292.05 Regular price$324.50
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Ships within 48 hours · Estimated delivery Jul 15 - Jul 20

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Description

fT3 (Free Triiodothyronine) ELISA KitThe fT3 (Free Triiodothyronine) ELISA immunoassay kit uses the Competitive ELISA principle. The micro ELISA plate provided in this kit has been pre coated with fT3. Standards or samples are added to the appropriate micro ELISA plate wells then with a biotin conjugated antibody specific to fT3. Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme

The fT3 (Free Triiodothyronine) ELISA immunoassay kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with fT3. Standards or samples are added to the appropriate micro ELISA plate wells then with a biotin-conjugated antibody specific to fT3. Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of fT3 in the samples is then determined by comparing the OD of the samples to the standard curve.

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SKU: 13407734266

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