SKU: 52167916180

17-OHP (17-Hydroxyprogesterone) ELISA Kit

Sale price$202.50 Regular price$225.00
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Ships within 48 hours · Estimated delivery Jul 20 - Jul 25

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Description

17-OHP (17-Hydroxyprogesterone) ELISA KitThe 17 OHP (17 Hydroxyprogesterone) ELISA immunoassay kit uses the Competitive ELISA principle. The micro ELISA plate provided in this kit has been pre coated with 17 OHP. Standards or samples are added to the appropriate micro ELISA plate wells then with a biotin conjugated antibody specific to 17 OHP. Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The

The 17-OHP (17-Hydroxyprogesterone) ELISA immunoassay kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with 17-OHP. Standards or samples are added to the appropriate micro ELISA plate wells then with a biotin-conjugated antibody specific to 17-OHP. Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 17-OHP in the samples is then determined by comparing the OD of the samples to the standard curve.

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SKU: 52167916180

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