Human FRS2 (Fibroblast Growth Factor Receptor SubstRate 2) ELISA Kit

Human FRS2 (Fibroblast Growth Factor Receptor SubstRate 2) ELISA KitThe Human FRS2 (Fibroblast Growth Factor Receptor SubstRate 2) ELISA Kit measures FRS2 in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti FRS2 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add

The Human FRS2 (Fibroblast Growth Factor Receptor SubstRate 2) ELISA Kit measures FRS2 in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody-Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti FRS2 antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody. Then, it binds with FRS2 bound to precoated antibody. Wash unbound components and add HRP-Streptavidin Conjugate (SABC). Wash unbound components again and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of FRS2 in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.