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Ships within 48 hours · Estimated delivery Jul 6 - Jul 11
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Description
cAMP OneStep competitive ELISA KitProduct Specification Antigen cAMP Immunogen Recombinant Protein Antibody Type Recombinant mAb Reactivity Others Purification Protein A Stability & Storage 12 months from date of receipt reconstitution, 2 to 8'C as supplied. Kit Precision Intra assay: 3. 6% Inter assay: 6. 1% Sample type Cell culture supernatant, Serum, Plasma; lysates Assay type Competitive Sensitivity 0. 199 pmoL mL Range 7. 59 pmoL mL 243 pmoL mL Recovery Cell culture supernatant:
Product Specification
| Antigen | cAMP |
| Immunogen | Recombinant Protein |
| Antibody Type | Recombinant mAb |
| Reactivity | Others |
| Purification | Protein A |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8'C as supplied. |
Kit
| Precision | Intra-assay: 3.6%； Inter-assay: 6.1% |
| Sample type | Cell culture supernatant, Serum, Plasma; lysates |
| Assay type | Competitive |
| Sensitivity | 0.199 pmoL/mL |
| Range | 7.59 pmoL/mL – 243 pmoL/mL |
| Recovery | Cell culture supernatant: 103% Serum: 110% Plasma: 102% |
| Assay time | 60 minutes |
| Species reactivity | Others |
Background
Cyclic adenosine monophosphate (cAMP) is a ubiquitous second messenger that plays a fundamental role in transducing extracellular signals into intracellular physiological responses. It is synthesized from ATP by adenylyl cyclase enzymes upon activation of G-protein-coupled receptors (GPCRs) and is degraded by phosphodiesterases (PDEs). The intracellular levels of cAMP directly regulate critical cellular processes, including metabolism, gene transcription, ion channel activity, cell proliferation, and apoptosis, primarily through the activation of protein kinase A (PKA) and the Exchange Protein directly Activated by cAMP (EPAC).
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