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Description
Phospho-ULK1 (Ser757) Recombinant Rabbit mAb (S-3495)Product Specification Host Rabbit Antigen Phospho ULK1 (Ser757) Synonyms Serine threonine protein kinase ULK1; Autophagy related protein 1 homolog (ATG1; hATG1); Unc 51 like kinase 1; KIAA0722; ULK1 Location Cytoplasm Accession O75385 Clone Number S 3495 Antibody Type Recombinant mAb Isotype IgG Application WB Reactivity Hu Positive Sample A172 Purification Protein A Concentration 0. 5 mg ml Conjugation Unconjugated Physical Appearance Liquid Storage
Product Specification
| Host | Rabbit |
| Antigen | Phospho-ULK1 (Ser757) |
| Synonyms | Serine/threonine-protein kinase ULK1; Autophagy-related protein 1 homolog (ATG1; hATG1); Unc-51-like kinase 1; KIAA0722; ULK1 |
| Location | Cytoplasm |
| Accession | O75385 |
| Clone Number | S-3495 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB |
| Reactivity | Hu |
| Positive Sample | A172 |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu |
Background
Phospho-ULK1 (Ser757) is the phosphorylated form of the serine/threonine kinase ULK1 at serine 757, a modification catalyzed by mTORC1 under nutrient-rich conditions that disrupts ULK1’s interaction with AMPK and keeps autophagy suppressed; upon starvation or rapamycin-triggered mTORC1 inhibition, Ser757 dephosphorylation enables AMPK binding and subsequent phosphorylation of activation-loop sites, licensing ULK1 kinase activity and initiating autophagosome formation.
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